R7638

Sigma

 

RPMI-1640 Medium

Dutch Modification, with sodium bicarbonate and 20mM HEPES, without L-glutamine, sterile-filtered, cell culture tested

MDL number:MFCD00217820

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Description

ApplicationRPMI 1640 Medium was developed at Roswell Park Memorial Institute in 1966 by Moore and his co-workers. A modification of McCoy's 5A Medium, it was formulated to support lymphoblastoid cells in suspension culture, but it has since been shown to support a wide variety of cells that are anchorage dependent. Originally intended to be used with a serum supplement, RPMI 1640 has been shown to support several cell lines in the absence of serum. It has also been widely used in fusion protocols and in the growth of hybrid cells.
ReconstitutionSupplement with 0.3 g/L L-glutamine.

Properties

sterilitysterile-filtered
total impurities Endotoxin, tested
suitabilitycell culture tested
storage temp.2-8°C

Safety

WGK Germany3